Raman spectroscopy is sensitive to biochemical changes related to various cartilage injuries

Raman spectroscopy is promising in vivo tool various biomedical applications; moreover, recent years, its use for characterizing articular cartilage degeneration has been developing. It also shown potential scoring the severity of lesions, which could be useful determining optimal treatment strategy during repair surgery. However, effect different injury types on spectra unknown. This study aims to investigate detecting changes due types. Artificial injuries were induced samples using established mechanical and enzymatic approaches mimic trauma-induced natural degeneration. Mechanical damage was surface abrasion (ABR, n = 12) or impact loading (IMP, 12), while three treatments: 30 min trypsin digestion (T30, 90 collagenase (C90, 24 h (C24, 12). obtained from all specimens, partial least squares discriminant analysis (PLS-DA) used distinguish their respective controls. PLS-DA cross-validation accuracies higher C24 (88%) IMP (79%) than C90 (67%), T30 (63%), ABR (58%) groups. indicates that spectroscopy, combined with multivariate analysis, can discern knowledge clinical decision-making, example, selecting remedy